Artificial Metalloenzymes for in vivo Catalysis: Challenges and Opportunities
Artificial metalloenzymes (ArMs) result from the incorporation of an abiotic metal cofactor within a host protein, Figure 1. Thanks to the remarkable supramolecular affinity of biotin for streptavidin (KD > 10-13 M), linking a biotin anchor to an organometallic catalyst ensures that, upon addition of streptavidin, the abiotic cofactor is…